CHRM4 antibody (AA 469-478)

Catalog No. ABIN233476

This Rabbit Polyclonal antibody specifically detects CHRM4 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CHRM4 antibody (AA 469-478) (ABIN233476)

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CHRM4 Antibody

Binding Specificity: AA 469-478

Specificity: Shown to be specific for the COOH terminal of the M4 receptor. Cross-reactivity: M4 Muscarinic Receptor (aa469-478): 100 % , M4 Muscarinic Receptor: ~70 %. No reactivity is observed with M1-M3, M5 (full receptors), M1 (aa451-460), M2 (aa457-466), M3 Muscarinic Receptor (aa580-589), M5 (aa519-531).

Purification: Ammonium sulfate precipitation

Immunogen: Peptide analogue of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC (50 μg/mL), WB

Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have been tested. Immunohistochemistry: 50 μg/mL stains PLP fixed cultured cells known to express the Muscarinic Receptor M4.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 10 mg/mL BSA, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CHRM4

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Alternative Name: CHRM4 / M4

Background: Name/Gene ID: CHRM4
Subfamily: Acetylcholine
Family: GPCR

Synonyms: CHRM4, M4 muscarinic receptor, M4R, Muscarinic m4 receptor, HM4, Muscarinic receptor m4

Gene ID: 1132

UniProt: P08173

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Synaptic Membrane